Purification and Partial Characterization of a New Antitumor Protein from Tegillarca granosa
نویسندگان
چکیده
A new protein, coded as D2-3, was obtained from the marine organism Tegillarca granosa L. by anion exchange and hydrophobic chromatography. The purity of D2-3 was over 99.0% as measured by RP-HPLC. Its molecular weight was shown to be 20.320 kDa by ESI-MS/MS, and the isoelectric point of D2-3 was 4.70. The antitumor activity of D2-3 against four human tumor cell lines was measured by MTT assay. The conformational structure of D2-3 was further characterized by UV-vis, FT-IR and CD spectroscopy. Partial amino acid sequences of D2-3 were determined to be LMMTDVEESR, SSHMLSECRRK, KNGRNVDISHKDKG, SSDPTLMDPDDTNKDR, SSDKNTCSKTEYYTR and SSETMPYDVLDTNEMR via MALDI-TOF-MS and de novo sequencing.
منابع مشابه
The impact of Tegillarca granosa extract haishengsu on HL-60 cell
Haishengsu (Hss) is a purified protein from Tegillarca granosa that has been used as a traditional Chinese medicine to treat cancer for more than a century. In this study, we observed the impact of Haishengsu (Hss) on the proliferation and differentiation of HL-60 cells in the leukemic cell line by taking tretinoin and AS2O3 as a positive control and making a comparative analysis between the ef...
متن کاملIdentification and Comparative Analysis of the Tegillarca granosa Haemocytes MicroRNA Transcriptome in Response to Cd Using a Deep Sequencing Approach
BACKGROUND MicroRNAs (miRNAs) are endogenous non-coding small RNAs (sRNAs) that can base pair with their target mRNAs, which represses their translation or induces their degradation in various biological processes. To identify miRNAs regulated by heavy metal stress, we constructed two sRNA libraries for the blood clam Tegillarca granosa: one for organisms exposed to toxic levels of cadmium (Cd)...
متن کاملPreliminary study on the growth development of blood cockle (Tegillarca granosa) by using different substrates in the hatchery system
Culturing of bivalves in the hatchery is the most popular technique for aquaculture that promotes a healthy bivalvechr('39')s production, where the animal would be less exposed to pollution from human activities. The increase of industrial and agricultural waste along the coastal region has altered the environment, hence affecting the growth and survival of aquatic organisms, particularly clams...
متن کاملLaser-Induced Breakdown Spectroscopy for Rapid Discrimination of Heavy-Metal-Contaminated Seafood Tegillarca granosa
Tegillarca granosa samples contaminated artificially by three kinds of toxic heavy metals including zinc (Zn), cadmium (Cd), and lead (Pb) were attempted to be distinguished using laser-induced breakdown spectroscopy (LIBS) technology and pattern recognition methods in this study. The measured spectra were firstly processed by a wavelet transform algorithm (WTA), then the generated characterist...
متن کاملIdentification of a regulation network in response to cadmium toxicity using blood clam Tegillarca granosa as model
Clam, a filter-feeding lamellibranch mollusk, is capable to accumulate high levels of trace metals and has therefore become a model for investigation the mechanism of heavy metal toxification. In this study, the effects of cadmium were characterized in the gills of Tegillarca granosa during a 96-hour exposure course using integrated metabolomic and proteomic approaches. Neurotoxicity and distur...
متن کامل